AACR-Aflac Incorporated Career Development Awards for Pediatric Cancer Research
The AACR Career Development Award for Pediatric Cancer Research is open to junior faculty in their first full-time faculty appointment. Proposed research projects may be in any discipline of basic, clinical, translational or epidemiological research with direct applicability and relevance to pediatric cancer.
Wei Li, PhD
Research Scientist, Sloan-Kettering Institute for Cancer Research, New York, NY
The Pro-oncogenic Role of EZH2 and CRL4(DCAF1) in NF2 Mutant Gliomas
Brain tumors are a major class of pediatric cancers. These tumors arise in the cranium or central spinal canal. Because of the limited space of the intracranial cavity, brain tumors are usually inherently serious and life-threatening. Despite major advances in neuroimaging and neurosurgical techniques over the past decades, the neurosurgical management of brain tumor patients remains challenging. In order to radically alter the clinical course of these brain tumors, it is important to develop targeted therapies based on identified oncogenic mutations and signaling pathways that drive their development and sustain their maintenance. Inactivating mutations in the NF2 tumor suppressor gene have been linked to gliomas and some other pediatric brain tumors. The NF2 gene encodes the FERM domain protein Merlin. The mechanisms by which Merlin suppresses tumorigenesis have long remained unclear, therefore hampering the progress in the development of targeted therapies for NF2 mutant tumors. Dr. Li has recently discovered that the active form of Merlin accumulates in the nucleus and inhibits an E3 Ubiquitin ligase CRL4DCAF1. Genetic epistasis experiments and analysis of several Merlin missense mutations from patients support the hypothesis that the dephosphorylated form of Merlin suppresses tumorigenesis by inhibiting CRL4DCAF1. Following this study, he identified the Lats kinases in the Hippo tumor suppressor pathway as substrates of CRL4DCAF1. By ubiquitylating and inhibiting Lats, CRL4DCAF1 activates the oncogenic transcription coactivators YAP. These findings identify the oncogenic elements of this newly discovered pathway, CRL4DCAF1 and YAP, as therapeutic targets in NF2 mutant tumors. He will use the support from the AACR-Aflac Inc. Career Development Awards for Pediatric Cancer Research to further study the inhibitory regulation of Lats by CRL4DCAF1. This study will focus on examining the role of a Polycomb-group methyltransferase EZH2 in facilitating the inhibitory regulation, and exploring the therapeutic efficacy when CRL4DCAF1 and EZH2 are inhibited in NF2 mutant glioma cells. If these studies determine that NF2 mutant glioma cells are sensitive to these inhibitions, it will provide scientific rationale to test if this method can be translated into novel clinical trials for patients with NF2 mutant gliomas.
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Yiping He, PhD
Assistant Professor/Pathology, Duke University, Durham, NC
Role of the MLL2-REST Link in Medulloblastoma Pathogenesis
"Medulloblastoma is the most common malignant brain tumor in children. Despite advances in therapy, about 30 percent of children afflicted with medulloblastoma die of the disease. Children who survive often suffer from severe side effects as a result of damage done to the developing brain caused by current standard treatment. To achieve our long-term goal of developing better therapeutic strategies that are both curative and without serious side effect, it is essential that we understand the underlying oncogenic alterations and the resulting consequences for cellular processes in medulloblastoma.
"Recently, a promising development arose showing that the Mixed-lineage leukemia 2 (MLL2) pathway is a frequently mutated driver in medulloblastoma and other cancers. MLL2 is a histone methyltransferase and tumorigenesis is thought to be driven by its inactivating mutations. However, the underlying mechanisms by which inactivation of MLL2 drives pathogenesis remain unknown. Studying MLL2 has been challenging due to the large size (~600 kDa) of the protein and the lack of appropriate in vitro and in vivo cancer models. To overcome these obstacles, we have established novel human cell-based models by using gene knockout and somatic genetic editing technologies. Functional assays of MLL2 in these models reveal that MLL2 regulates a variety of signaling pathways including those essential for cell cycle progression and cell differentiation. The objective of our continuous research is to illuminate the role of signaling pathways that mediate MLL2 inactivation-driven tumorigenesis. In particular, we will use the AACR-Aflac Inc. Career Development Award for Pediatric Cancer Research to determine the role of MLL2 in medulloblastoma cell differentiation, the effect of MLL2 inactivation on REST, another regulator of cell differentiation, and to uncover other downstream mediators of MLL2’s function. Findings from these studies will contribute to our understanding of medulloblastoma and improving therapeutics. The generous support provided by the 2013 AACR-Aflac Inc. Career Development Award for Pediatric Cancer Research will, therefore, move us one step closer to our long-term goal of developing better medulloblastoma therapeutic strategies."