AACR-John and Elizabeth Leonard Family Foundation Basic Cancer Research Fellowship
The AACR-John and Elizabeth Leonard Family Foundation Basic Cancer Research Fellowships represent a joint effort to encourage and support mentored young investigators to conduct basic cancer research and to establish a successful career path in this field. Eligibility is limited to postdoctoral and clinical research fellows who will have completed their most recent doctoral degree within the past three years. The research proposed for funding may be in any area of basic cancer research.
Ruli Gao, PhD
University of Texas MD Anderson Cancer Center
Tracking stromal interactions in TNBC chemoresistance by single cell RNAseq
Scientific Statement of Research
Due to lack of hormonal receptors, triple-negative breast cancer (TNBC) patients are usually treated with neoadjuvant chemotherapy followed by surgery/radiation therapy. However the majority of patients evolved resistance, often leaving no further treatment options. To date, the genetic and molecular mechanisms of chemo-resistance in TNBC patients are still poorly understood.
Dr. Gao hypothesized that chemo-resistant subclones are pre-existing in primary tumors with mesenchymal phenotypes and are maintained through stromal interactions. She will investigate tumor cell plasticity, tumor-stroma interactions, and their roles in TNBC chemo-resistance evolution by unbiased profiling thousands of single cell transcriptomes. Dr. Gao’s studies will reveal the transcriptional basis of chemo-resistance in TNBC patients, and will identify stromal cell types that play important roles in this process. Her research is expected to identify therapeutic targets in both the tumor and stromal cells that can be exploited to overcome chemo-resistance, and will, therefore, greatly benefit resistant TNBC patients.
Dr. Gao received her bachelor's degree in biotechnology and a master's degree in biochemistry and molecular biology from Yangzhou University in China. She then came to United States, and received a second master's degree in statistics and a doctoral degree in genetics and genomics with Dr. Frederic Kaye from University of Florida in 2014. To further pursue trainings in single cell genomic researches, she moved the UT MD Anderson Cancer Center to work with Dr. Nicholas Navin, where she studied the punctuated copy number evolution in triple-negative breast cancer using single cell DNA sequencing methods. Her current researches are migrating to tracing chemo-resistance in triple negative breast cancer by delineating single cell transcriptomes. To perform these studies, she and colleagues have developed a high throughput nanogrid single nuclei RNAseq method to profile thousands of single cells at low costs (~$1 per cell). Her long-term goal is to become a statistical geneticist to understand how genomic and phenotypic diversity play roles in tumor evolution and therapy resistance in cancer patients.
Acknowledgement of Support
I sincerely thank the generous funding support from the AACR Basic Cancer Research Fellowship program. This prestigious fellowship will greatly help my researches and promote my career success by expanding my knowledge and expertise in cancer genomic researches, and allowing me to become an active member of AACR research community.
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